LeXso HotSpot Panel v1.0
- #LX11402
- #LX11401
The LeXso HotSpot Panel v1.0 is a precision-engineered targeted enrichment solution designed for the exhaustive analysis of high-frequency tumor mutation “hotspots”. By targeting 49 essential genes involved in oncogenesis and tumor suppression, this panel provides a focused 25 Kb genomic footprint, enabling ultra-high-depth sequencing for the accurate detection of low-frequency variants in both tissue and liquid biopsy samples.
Optimized for the LeXso Hybrid System
Specifically designed for use with the LeXso Hybrid Capture System, the HotSpot Panel v1.0 leverages our novel targeted enrichment technology to deliver superior results. The integration of high-density probes and rapid-capture kinetics ensures:
- Improved Capture Efficiency: Maximizes the detection of rare alleles while minimizing sequencing waste.
- Consistent & Excellent Performance: Delivers high uniformity and stable capture across diverse sample types.
- Accelerated Timelines: Significantly reduces experimental time, enabling more efficient laboratory operations and faster time-to-insight.
- Product Details
- Kit Content
- FAQ
- Ordering Information
- Resources
Precision Gene Curation
- Focused analysis of 49 genes known to contain critical mutational hotspots, ensuring that sequencing resources are concentrated on the most actionable genomic regions.
Enhanced Sequencing Economy
- The strategic 25 Kb design allows for deep coverage (10,000x or greater) at a fraction of the cost of larger exome-scale or comprehensive oncology panels.
Rapid Targeted Enrichment
- Optimized for speed, the panel allows laboratories to complete the entire hybridization and capture workflow in a single afternoon when paired with LeXso rapid-capture reagents.
Superior Data Uniformity
- Advanced probe architecture ensures equitable representation of all targeted regions, providing the high-fidelity data needed for precise variant calling in challenging samples.
Cost-Effective Scalability
- High on-target rates and reduced manual hands-on time lead to significant operational savings, making it an ideal choice for high-throughput screening and longitudinal research.
| AKT1 | ALK | APC | AR | ARAF | ATM | BRAF | CDKN2A | CHEK2 | CTNNB1 |
| DDR2 | EGFR | ERBB2 | ERBB3 | ESR1 | FBXW7 | FGFR1 | FGFR2 | FGFR3 | FGFR4 |
| FLT3 | GNA11 | GNAQ | GNAS | HRAS | IDH1 | IDH2 | KIT | KRAS | MAP2K1 |
| MAP2K2 | MET | MTOR | NRAS | NTRK1 | NTRK3 | PDGFRA | PIK3CA | PTCH1 | PTEN |
| RAF1 | RET | ROS1 | SF3B1 | SMAD4 | SMO | STK11 | TP53 | TSC1 |
| Catalog | Color Of Tube Cap | Component | Volume | Package/Storage |
| LX11402 | ● | LeXso HotSpot Panel v1.0, 16 rxn | 40 μL | -20℃ |
| LX11401 | ● | LeXso HotSpot Panel v1.0, 96 rxn | 210 μL | -20℃ |
Yes. For the highest data quality, we recommend using the LeXPrep DNA Library Preparation Module v2. This combination is optimized to provide the high library complexity and conversion efficiency necessary for the high-depth sequencing required to identify low-frequency hotspot mutations.
Absolutely. The focused design and the ultra-high sensitivity of the LeXso Hybrid Capture System make this panel particularly effective for detecting trace amounts of circulating tumor DNA (ctDNA) in blood-based samples, facilitating non-invasive monitoring of tumor dynamics.
Traditional hybridization can take 16–24 hours. The LeXso HotSpot Panel, when used with LeXso reagents, utilizes optimized kinetics that allow for high-performance hybridization in as little as 1–2 hours, effectively bypassing the overnight bottleneck.
The panel is specifically designed for the high-sensitivity detection of Single Nucleotide Variants (SNVs) and small Insertions/Deletions (Indels) within the targeted 49-gene hotspot regions.
| Product | Catalog |
|---|---|
| LeXso HotSpot Panel v1.0, 16 rxn | LX11402 |
| LeXso HotSpot Panel v1.0, 96 rxn | LX11401 |
For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com