LeXPrep Single Cell WGA Kit
- #LX30101
- #LX30102
LeXPrep Single Cell WGA Kit: High-Fidelity Whole Genome Amplification
The LeXPrep Single Cell WGA Kit is a high-performance solution engineered for the robust whole-genome amplification (WGA) of genomic DNA from single cells or ultra-low input samples. Utilizing a proprietary system of optimized buffers and high-fidelity enzymes, this kit enables researchers to move from raw cells to microgram-level gDNA in just 3 hours.
Optimized for Precision and Yield
The kit features a streamlined, three-step workflow—Cell Lysis, Preamplification, and Amplification—designed to generate high-quality genomic material with exceptional coverage and uniformity. By minimizing sequence bias, the LeXPrep system ensures the highest accuracy for downstream variant calling and structural analysis.
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- Ultra-Low Input Sensitivity: Specifically designed for single or multiple cells (1–10) or trace amounts of purified gDNA (>30 pg), making it ideal for rare cell analysis and precious samples.
- Enhanced Data Quality: Significant improvements in sequencing coverage and genomic uniformity facilitate precise detection of SNPs, Indels, and Copy Number Variations (CNVs).
- Automation-Ready Simplicity: Automation thrives on simplicity; the kit’s streamlined, low-intervention protocol is perfectly suited for integration into automated workstations, reducing the potential for human error and ensuring repeatable, robust results.
- Broad Downstream Compatibility: Fully validated for a wide range of applications, including Whole Genome Sequencing (WGS), large-fragment CNV analysis, and quantitative PCR (qPCR).
With a total processing time of 180 minutes, the LeXPrep Single Cell WGA Kit removes the traditional bottlenecks of single-cell genomics, providing a fast, reliable, and scalable bridge from ultra-low input material to comprehensive genomic insights.
| Package | Color Of Tube Cap | Component | Volume (24 rxn) | Volume (96 rxn) |
| Box 1 | ●● | Cell Lysis Buffer | 130 μL | 520 μL |
| ● | Cell Lysis Enzyme | 16 μL | 65 μL | |
| ● | 1st Amplification Buffer | 320 μL | 1,270 μL | |
| ● | 1st Amplification Enzyme | 32 μL | 130 μL | |
| ● | 2nd Amplification Buffer | 810 μL | 2×1,615 μL | |
| ● | 2nd Amplification Enzyme | 65 μL | 250 μL | |
| / | LeXPrep SP Beads | 2.5 mL | 8 mL |
This is typically attributed to two main factors:
- Cell Loss: If cells are lost during isolation or transfer, amplification will fail. We recommend optimizing your cell collection workflow and confirming the presence of a cell in the reaction tube before starting lysis.
- Sample Impurity: Residual wash buffers, ethanol, or other contaminants from purification can inhibit the enzymatic reaction. Ensure ethanol is completely evaporated and resuspend purified gDNA in Nuclease-Free Water. Always verify input quantification using high-sensitivity methods.
High yield does not always guarantee high genomic integrity. These artifacts are usually caused by template degradation:
- For Cell Inputs: Cell apoptosis prior to lysis leads to DNA fragmentation. Always assess cell viability before processing. If immediate amplification isn’t possible, store lysed cells at -20°C (±5°C) for no more than 7 days to preserve integrity.
- For Bulk DNA Inputs: Partially degraded starting material will produce uneven coverage. Ensure you are using high-molecular-weight, intact gDNA, or consider increasing the initial input amount to compensate for lower quality.
Because the LeXPrep system is extremely sensitive, even trace amounts of exogenous DNA can lead to microgram-level amplification in blanks. To mitigate this:
- Environment: Perform all pre-amplification steps in a dedicated, DNA-free cleanroom or laminar-flow hood.
- Consumables: Use only certified nuclease-free and DNA-free tips and tubes.
- Decontamination: Regularly treat work surfaces and pipettes with DNA-degrading solutions and UV light. Always include and monitor negative controls to quickly identify contamination sources.
Yes. Due to its superior amplification uniformity and low Allele Dropout (ADO) rates, the kit is an excellent choice for detecting chromosomal mosaicism and fine-scale copy number abnormalities in single-cell samples.
The LeXPrep Single Cell WGA Kit has been validated across a diverse range of mammalian cell types, including blastomeres, trophectoderm cells, and various cultured cell lines, ensuring consistent performance regardless of your research model.
| Product | Catalog |
|---|---|
| LeXPrep Single Cell WGA Kit, 24 rxn | LX30101 |
| LeXPrep Single Cell WGA Kit, 96 rxn | LX30102 |
For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com