LeXPrep rRNA Blocking Reagent

Catalog Number:
  • #LX02901
  • #LX02902
  • #LX02911
  • #LX02912
  • #LX02921
  • #LX02922
  • #LX02931
  • #LX02932
  • #LX02941
  • #LX02942
  • #LX02951
  • #LX02952
  • #LX029XX

LeXPrep rRNA Blocking Reagent: High-Efficiency Depletion for NGS

The LeXPrep rRNA Blocking Reagent is a specialized solution engineered for high-throughput sequencing platforms, designed to rapidly and effectively deplete ribosomal RNA (rRNA) from total RNA samples. By streamlining the removal of unwanted transcripts, this module enhances sequencing depth for target sequences and improves overall data quality.

 

 

  • Product Details
  • Kit Content
  • FAQ
  • Ordering Information
  • Resources
Product Features
  • Species-Specific Versatility: Available in both standard commercial kits and fully customized designs tailored to your specific research organism.
  • Broad Sample Compatibility: Optimized for a wide range of input amounts (50–500 ng) and diverse sample types, including high-quality cells, tissues, and challenging FFPE samples.
  • Rapid Workflow: Specifically developed to meet the demands of high-throughput labs, significantly reducing processing time without compromising depletion efficiency.
Workflow

Performance

Custom Solutions & Consultation

We provide personalized, professional advice for researchers requiring custom-designed probes for unique species. To discuss your project or request a quotation, please send your request to our technical team at support@lexigenbio.com

For research use only. Not for use in diagnostic procedures.

CatalogColor Of Tube CapProductVolumePackage/Storage
LX02901LeXPrep rRNA Blocking Reagent (H), 24 rxn30 μL−20°C
LX02902LeXPrep rRNA Blocking Reagent (H), 96 rxn115μL−20°C
LX02911LeXPrep rRNA Blocking Reagent (Zebrafish), 24 rxn30 μL−20°C
LX02912LeXPrep rRNA Blocking Reagent (Zebrafish), 96 rxn115μL−20°C
LX02921LeXPrep rRNA Blocking Reagent (HMR), 24 rxn30 μL−20°C
LX02922LeXPrep rRNA Blocking Reagent (HMR), 96 rxn115μL−20°C
LX02931LeXPrep rRNA Blocking Reagent (Plant), 24 rxn30 μL−20°C
LX02932LeXPrep rRNA Blocking Reagent (Plant), 96 rxn115μL−20°C
LX02951LeXPrep rRNA Blocking Reagent (Bacteria), 24 rxn30 μL−20°C
LX02952LeXPrep rRNA Blocking Reagent (Bacteria), 96 rxn115μL−20°C
LX02941LeXPrep Globin mRNA Blocking Reagent, 24 rxn30 μL−20°C
LX02942LeXPrep Globin mRNA Blocking Reagent, 96 rxn115μL−20°C
LX029XXLeXPrep rRNA Blocking Reagent (Custom), 96 rxn115μL−20°C

 

Q1. Is the LeXPrep rRNA Fast Blocking Comprehensive Solution suitable for severely degraded FFPE samples?

A: This solution is compatible with RNA samples from a wide range of cell and tissue sources across varying degradation levels. However, it is not recommended for severely degraded FFPE samples where the DV200 is less than 20%.

Q2. Is the LeXPrep rRNA Fast Blocking Comprehensive Solution compatible with third-party RNA library preparation kits?

A: Yes. This solution can be seamlessly integrated into various RNA-Seq library preparation workflows. If opting for a third-party kit, we specifically recommend the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB, Cat # E7770L) or the KAPA RNA HyperPrep Kit (Roche, Cat # KK8541). For inquiries regarding other brands, please consult the technical support team at support@lexigenbio.com

Q3. Why is the dsDNA yield occasionally higher when using the LeXPrep rRNA Fast Blocking Comprehensive Solution?

A: This solution utilizes a high concentration of rRNA ssDNA blocking reagents. When used alongside the LeXPrep Total RNA-To-DNA Module, trace amounts of residual ssDNA may remain in the purified dsDNA product. Because nucleic acid fluorescence quantifiers (like Qubit) can be influenced by residual ssDNA, the reported yield may appear slightly elevated. However, this does not interfere with downstream applications. We recommend adjusting adapter dilution factors and PCR cycles based on your specific RNA source and input amount.

ProductCatalog
LeXPrep rRNA Blocking Reagent (Human), 24 rxnLX02901
LeXPrep rRNA Blocking Reagent (Human), 96 rxnLX02902
LeXPrep rRNA Blocking Reagent (Zebrafish), 24 rxnLX02911
LeXPrep rRNA Blocking Reagent (Zebrafish), 96 rxnLX02912
LeXPrep rRNA Blocking Reagent (HMR), 24 rxnLX02921
LeXPrep rRNA Blocking Reagent (HMR), 96 rxnLX02922
LeXPrep rRNA Blocking Reagent (Plant), 24 rxnLX02931
LeXPrep rRNA Blocking Reagent (Plant), 96 rxnLX02932
LeXPrep rRNA Blocking Reagent (Bacteria), 24 rxnLX02951
LeXPrep rRNA Blocking Reagent (Bacteria), 96 rxnLX02952
LeXPrep Globin mRNA Blocking Reagent, 24 rxnLX02941
LeXPrep Globin mRNA Blocking Reagent, 96 rxnLX02942
LeXPrep rRNA Blocking Reagent (Custom), 96 rxnLX029XX

For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com

Product Sheet

  • 1. Brochure

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