LeXPrep RNA & DNA Library DualPrep Module
- #LX02411
- #LX02412
LeXPrep RNA & DNA Library DualPrep Module: Synchronous Metagenomic Library Construction
The LeXPrep RNA & DNA Library DualPrep Module is a high-performance solution engineered for the synchronous preparation of both RNA and DNA libraries from a single sample. Specifically optimized for pathogenic microbial samples, this integrated module eliminates the need for separate workflows, providing a comprehensive genomic and transcriptomic snapshot of complex mixed populations.
Integrated “Sample-to-Sequencer” Workflow
The DualPrep Module consolidates multiple complex steps into a streamlined, single-tube process, including:
- 1st & 2nd Strand cDNA Synthesis
- DNA Fragmentation
- End Repair & A-Tailing
- Adapter Ligation & Library Amplification
This module is designed for universal compatibility and performs seamlessly with the LeXPrep Universal Stubby Adapter (UDI) Module and LeXPrep Universal Adapter (MDI) Module for MGI, ensuring a flexible path to results on any major sequencing platform.
- Product Details
- Kit Content
- FAQ
- Ordering Information
- Resources
Flexible Multi-Analyte Compatibility
- Supports a wide range of input ratios, optimized for 10–100 ng RNA and 1–100 ng DNA.
- Fully compatible with both Illumina and MGI sequencing ecosystems.
Rapid Single-Tube Operation
- Minimize hands-on time and sample loss with a unified, single-tube protocol.
- Complete the entire RNA & DNA co-preparation process in just 3–3.5 hours.
Unmatched Efficiency and Uniformity
- Delivers high-stability yields even with challenging microbial samples.
- Ensures uniform fragment size distribution and exceptionally low GC bias for equitable genomic representation.
Advanced Pathogen Contamination Control
- Engineered for the rigorous demands of mNGS, featuring comprehensive background control of pathogenic microorganisms across the entire manufacturing and quality control process.
| Package | Color Of Tube Cap | Component | Volume (24 rxn) | Volume (96 rxn) |
| Box 1 | ● | Co-Random Primer | 60 µL | 230 µL |
| ● | 1st Co-Strand Synth. Buffer | 120 µL | 460 µL | |
| ● | 1st Co-strand Synth.Enzyme | 60 µL | 230 µL | |
| ● | 2nd Co-strand Synth.Buffer | 240 µL | 960 µL | |
| ● | 2nd Co-strand Synth. Enzyme | 60 µL | 230 µL | |
| ● | Co-FERA Buffer | 150 µL | 575 µL | |
| ● | Co-FERA Enzyme | 150 μL | 575 µL | |
| ● | Ligation Buffer | 750 μL | 2×1500 μL | |
| ● | DNA Ligase | 65 µL | 250 µL | |
| ● | 2X HiFi PCR Master Mix | 720 µL | 2×1,600 µL | |
| / | Nuclease Free Water | 2.5 mL | 8 mL | |
| ● | TE Solution | 1 mL | 4 mL | |
| Box 2 | / | LeXPrep SP Beads | 4 mL | 15 mL |
If you are using co-extracted total nucleic acids, ensure you quantify RNA and DNA separately using high-sensitivity methods (e.g., Qubit) and follow the recommended PCR cycles for your specific input. Additionally:
- Environment: Use strictly RNase-free consumables and workspaces to maintain RNA integrity.
- Purification: If samples contain high levels of inhibitors (guanidine salts, phenol, ethanol), we recommend a pre-prep cleanup using 2X LeXPrep SP Beads and eluting in Nuclease-Free Water.
- Optimization: For lower-quality or degraded samples, consider increasing the PCR cycle count by 1–2 cycles.
This is often caused by residual EDTA or an incorrect pH level inhibiting enzymatic digestion. We recommend purifying your starting material with 2X LeXPrep SP Beads and replacing the elution buffer with Nuclease-Free Water to ensure optimal enzyme activity.
Yes. This module can tolerate samples containing up to 6 μL of 1 mM EDTA (resulting in a final concentration of 0.3 mM in the 1st strand synthesis). If your sample solvent exceeds this threshold, a bead-based cleanup is necessary before proceeding.
Yes. When co-preparing libraries from mixed samples, a higher DNA-to-RNA ratio may result in slightly larger fragment lengths during enzymatic digestion. You can easily compensate for this by adjusting the fragmentation time according to the insert lengths recommended in the manual.
Absolutely. Libraries prepared with this module are fully compatible with the LeXso Hybrid Capture Reagents. This integration significantly shortens the turnaround time for pathogen-targeted sequencing and simplifies the experimental burden.
The LeXPrep DualPrep Module is engineered to match the performance of our specialized DNA kits. Benchmarking against the LeXPrep EZ DNA Library Preparation Module v2 shows nearly identical library yields, mappability, and capture performance, ensuring you don’t sacrifice DNA data quality when choosing a dual-analyte workflow.
| Product | Catalog |
|---|---|
| LeXPrep RNA & DNA Library DualPrep Module, 24 rxn | LX02411 |
| LeXPrep RNA & DNA Library DualPrep Module, 96 rxn | LX02412 |
For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com