LeXPrep Methyl Library Preparation Module
- #LX02511
- #LX02512
LeXPrep Methyl Library Preparation Kit v2: High-Performance Epigenetic Profiling
The LeXPrep Methyl Library Preparation Kit v2 is a sophisticated solution engineered for the construction of high-quality methylation sequencing (Methyl-Seq) libraries from double-stranded DNA (dsDNA). Optimized for both Illumina and MGI platforms, this kit provides researchers with the high-fidelity tools necessary to uncover the complex epigenetic landscape with precision and efficiency. Whether performing Whole-Genome Bisulfite Sequencing (WGBS) or hybrid capture-based targeted methylation sequencing, the LeXPrep v2 kit delivers consistent, high-resolution data. It is designed to work seamlessly with our LeXPress hybrid capture system, ensuring superior enrichment of targeted methylated regions.
- Product Details
- Kit Content
- FAQ
- Ordering Information
- Resources
- Broad Input Sensitivity (1–500 ng): Successfully process a wide range of input amounts across diverse DNA sample types, allowing for maximum utilization of precious or limited biological specimens.
- Universal Compatibility: Offering total flexibility for your lab’s ecosystem, the kit is compatible with multiple conversion methodologies (including bisulfite conversion) and all major sequencing platforms.
- Rapid Workflow: Streamlined chemistry enables the completion of WGBS library preparation—including conversion—in as little as 4.5 hours, significantly reducing time-to-results.
- Enhanced Data Utilization: Optimized library complexity and conversion efficiency significantly improve effective sequencing depth, ensuring more actionable data per run and reduced sequencing costs.
| Package | Color Of Tube Cap | Component | Volume (24 rxn) | Volume (96 rxn) |
| Box 1 | ● | EndRepair & A-Tailing Buffer Pro | 180 µL | 690 µL |
| ● | End Repair & A-Tailing Enzyme Pro | 120 µL | 460 µL | |
| ● | Ligation Buffer | 750 µL | 2×1,500 µL | |
| ● | DNA Ligase | 65 µL | 250 µL | |
| ● | 2X HiFi-Methyl PCR Master Pro Mix | 720 µL | 2×1,600 µL | |
| / | Nuclease Free Water | 2.5 mL | 8 mL | |
| ● | TE Solution | 1 mL | 4 mL | |
| Box 2 | / | LeXPrep SP Beads | 4 mL | 15 mL |
Yes. While optimized for high-performance bisulfite workflows, the library preparation chemistry is flexible enough to be compatible with various enzymatic or chemical conversion methods, allowing you to choose the best approach for your specific research goals.
Absolutely. The LeXPrep v2 kit is engineered to handle challenging sample types, including fragmented DNA from FFPE tissue. When paired with our high-uniformity probes, it provides reliable methylation profiling even from degraded starting material.
By minimizing DNA loss during the conversion and library prep steps, the kit maintains high library complexity. This results in fewer PCR duplicates and higher unique alignment rates, allowing you to reach your required coverage depth with less total sequencing.
| Product | Catalog |
|---|---|
| LeXPrep Methyl Library Preparation Module v2, 24 rxn | LX02511 |
| LeXPrep Methyl Library Preparation Module v2, 96 rxn | LX02512 |
For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com