LeXPrep IGTR Multiplex PCR Library Prep Kit
- #LX30001
- #LX30002
LeXPrep IGTR Multiplex PCR Library Prep Kit utilizes multiplex amplification to efficiently enrich the complementarity-determining regions (CDR3) across seven human adaptive immune receptor loci: IGH, IGK, IGL, TRA, TRB, TRG, and TRD. Paired with high-throughput sequencing, this kit enables high-resolution detection of B-cell and T-cell diversity and clonality. It is compatible with both DNA and cDNA from diverse sources, making it a versatile solution for immune repertoire profiling, lymphoid malignancy analysis, and minimal residual disease (MRD) monitoring.
- Product Details
- Kit Content
- FAQ
- Ordering Information
- Resources
Modular Primer Design
- Dual-Independent Primer Sets: Features two specialized mixes: the IG Primer Mix (covering IGH, IGL, and IGK) and the TR Primer Mix (covering TRA, TRB, TRG, and TRD).
- Flexible Detection: Supports single-tube independent analysis (IG or TR only) or dual-tube combined detection (IG + TR) for comprehensive profiling.
Broad Compatibility
- Sample Versatility: Compatible with human gDNA or first-strand cDNA (generated from RNA via reverse transcription).
- Input Flexibility: Supports a wide range of input amounts, from 50 ng to 2,000 ng.
- Platform Agnostic: Seamlessly integrates with LeXPrep workflows for direct sequencing on Illumina® platforms or MGI platforms (following circularization).
For research use only. Not for use in diagnostic procedures.
| Catalog | Color Of Tube Cap | Product | Volume (6 rxn) | Volume (96 rxn) |
| Box 1 | ● | 2X Multiplex PCR Mix | 180 μL | 2×1,600 μL |
| ● | IG Primer Mix | 15μL | 230 μL | |
| ● | TR Primer Mix | 15μL | 230 μL | |
| ● | Multiplex Index Primer Mix # | 6×5 μL | 24×10 μL | |
| Box 2 | / | LeXPrep SP Beads | 400 μL | 6,400 μL |
A: No. This kit is compatible only with gDNA or cDNA. For RNA samples, reverse transcription to generate cDNA is required prior to starting the library preparation protocol.
A: The LeXPrep kit supports an input range of 50–2,000 ng of gDNA or cDNA. If the input amount exceeds 2,000 ng, we recommend splitting the sample into multiple reactions to maintain optimal amplification efficiency.
A: The primary peak of PCR products generated by the LeXPrep kit is approximately 270 bp. We recommend PE150 (paired-end 150 bp) sequencing to ensure high-quality data coverage.
A: A minimum of 0.3 Gb of data is recommended to detect clones at 0.01% abundance with a 200 ng input. To enhance sensitivity for lower-frequency clones, the data volume should be increased accordingly.
A: Yes. The kit includes IG and TR Primer Mixes provided in separate tubes, allowing for flexible combination in a single amplification reaction. Due to its high sensitivity and ability to detect low-frequency variants, LeXPrep is an ideal solution for MRD monitoring technology development and clinical research applications.
| Product | Catalog |
|---|---|
| LeXPrep IGTR Multiplex Library Prep Kit, 6 rxn | LX30001 |
| LeXPrep IGTR Multiplex Library Prep Kit, 96 rxn | LX30002 |
For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com