LeXPrep EZ DNA Library Preparation Kit v2/v3 is engineered to generate high-quality sequencing libraries from double-stranded DNA (dsDNA) for all mainstream sequencing platforms. By optimizing the enzymatic fragmentation system, this kit maximizes library yield and complexity while maintaining exceptionally low background noise.
Designed for versatility, LeXPrep is compatible with various sample types—including gDNA and FFPE DNA—and is specifically fine-tuned for ultra-low-quality FFPE samples to ensure consistent, stable yields. To streamline your workflow, the fragmentation, end repair, and A-tailing processes are integrated into a single efficient step. This A-T ligation-based kit supports DNA inputs from 5 ng to 500 ng and is ideal for both Whole Genome Sequencing (WGS) and hybridization capture-based targeted sequencing.
Broad Sample Compatibility Highly suitable for diverse sample types, including genomic DNA (gDNA) and FFPE DNA of various qualities (Grades B+ through D).
Streamlined Workflow A flexible and user-friendly system where enzymatic fragmentation, end repair, and A-tailing are completed in a single-step reaction.
Controllable Fragmentation Offers precise control over DNA fragment length, significantly enhancing enzymatic recovery and library complexity.
Superior Accuracy & Low Noise Maintains ultra-low background noise (minimizing artifacts introduced by enzymatic digestion), even with low-quality samples. This reduces false positives and ensures high-accuracy variant detection.
Automation-Ready Design A flexible reaction chemistry with excellent automation compatibility, seamlessly integrating with NGS workstations to minimize manual labor and increase throughput.


| Package | Color Of Tube Cap | Component | Volume (24 rxn) | Volume (96 rxn) |
| Box 1 | ● | FERA Buffer III | 300 µL | 1200 µL |
| ● | FERA Enzyme III | 150 µL | 575 µL | |
| ● | Enhancer Buffer | 250 µL | 950 µL | |
| ● | Ligation Buffer III | 750 µL | 2×1,500 µL | |
| ● | DNA Ligase III | 65 µL | 250 µL | |
| ● | 2X HiFi PCR Master Pro Mix | 720 µL | 2×1,600 µL | |
| / | Nuclease Free Water | 2.5 mL | 8 mL | |
| ● | TE Solution | 1 mL | 4 mL | |
| Box 2 | / | LeXPrep SP Beads | 4 mL | 15 mL |
| Product | Catalog |
|---|---|
| LeXPrep EZ DNA Library Preparation Module v2, 24 rxn | LX02601 |
| LeXPrep EZ DNA Library Preparation Module v2, 96 rxn | LX02602 |
| LeXPrep EZ DNA Library Preparation Module v3, 24 rxn | LX02611 |
| LeXPrep EZ DNA Library Preparation Module v3, 96 rxn | LX02612 |
For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com