LeXPrep EZ DNA Library Preparation Module

Catalog Number:
  • #LX02601
  • #LX02602
  • #LX02611
  • #LX02612

LeXPrep EZ DNA Library Preparation Kit v2/v3 is engineered to generate high-quality sequencing libraries from double-stranded DNA (dsDNA) for all mainstream sequencing platforms. By optimizing the enzymatic fragmentation system, this kit maximizes library yield and complexity while maintaining exceptionally low background noise.

Designed for versatility, LeXPrep is compatible with various sample types—including gDNA and FFPE DNA—and is specifically fine-tuned for ultra-low-quality FFPE samples to ensure consistent, stable yields. To streamline your workflow, the fragmentation, end repair, and A-tailing processes are integrated into a single efficient step. This A-T ligation-based kit supports DNA inputs from 5 ng to 500 ng and is ideal for both Whole Genome Sequencing (WGS) and hybridization capture-based targeted sequencing.

  • Product Details
  • Kit Content
  • Ordering Information
  • Resources
Features

Broad Sample Compatibility Highly suitable for diverse sample types, including genomic DNA (gDNA) and FFPE DNA of various qualities (Grades B+ through D).

Streamlined Workflow A flexible and user-friendly system where enzymatic fragmentation, end repair, and A-tailing are completed in a single-step reaction.

Controllable Fragmentation Offers precise control over DNA fragment length, significantly enhancing enzymatic recovery and library complexity.

Superior Accuracy & Low Noise Maintains ultra-low background noise (minimizing artifacts introduced by enzymatic digestion), even with low-quality samples. This reduces false positives and ensures high-accuracy variant detection.

Automation-Ready Design A flexible reaction chemistry with excellent automation compatibility, seamlessly integrating with NGS workstations to minimize manual labor and increase throughput.

Workflow

Performance

PackageColor Of Tube CapComponentVolume (24 rxn)Volume (96 rxn)
Box 1FERA Buffer III300 µL1200 µL
FERA Enzyme III150 µL575 µL
Enhancer Buffer250 µL950 µL
Ligation Buffer III750 µL2×1,500 µL
DNA Ligase III65 µL250 µL
2X HiFi PCR Master Pro Mix720 µL2×1,600 µL
/Nuclease Free Water2.5 mL8 mL
TE Solution1 mL4 mL
Box 2/LeXPrep SP Beads4 mL15 mL

ProductCatalog
LeXPrep EZ DNA Library Preparation Module v2, 24 rxnLX02601
LeXPrep EZ DNA Library Preparation Module v2, 96 rxnLX02602
LeXPrep EZ DNA Library Preparation Module v3, 24 rxnLX02611
LeXPrep EZ DNA Library Preparation Module v3, 96 rxnLX02612

For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com

Product Sheet

  • 1. Brochure v3
  • 2. Brochure v2

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