LeXPrep DNA Library Preparation Module v2
- #LX02421
- #LX02422
LeXPrep DNA Library Preparation Module v2: Next-Generation Efficiency for Illumina & MGI Platforms
The LeXPrep DNA Library Preparation Module v2 is a high-performance solution engineered for the construction of superior-quality NGS libraries from double-stranded DNA (dsDNA). Utilizing a robust A-T ligation-based chemistry, this kit provides a stable and efficient foundation for a wide array of applications, from Whole Genome Sequencing (WGS) with 1–500 ng of input to ultra-sensitive hybridization capture-based targeted sequencing.
The v2 Advantage: High Complexity, Superior Fidelity
Building on the success of the original series, the upgraded v2 kit features significant advancements in library conversion efficiency. Specifically designed to handle the challenges of clinical research, the v2 module offers enhanced tolerance for low-quality samples, effectively increasing library complexity while maintaining the high fidelity required for precise variant calling.
- Product Details
- Kit Content
- FAQ
- Ordering Information
- Resources
- Fast and Convenient Workflow
Accelerate your laboratory throughput by completing the entire whole-genome library preparation process in under 3 hours.
- Stable and Efficient Performance
- Higher Library Yield: Optimized chemistry maximizes the recovery of DNA fragments.
- Superior Conversion Rates: More of your starting material is successfully converted into sequencing-ready libraries.
- Exceptional Complexity: Higher diversity in your libraries ensures better coverage of rare variants.
- Low GC Bias: Maintains equitable representation across genomic regions with varying GC content for more uniform data.
- Flexible Compatibility
- Multi-Platform Support: Seamlessly switch between MGI and Illumina platforms with a flexible selection of adapter modules.
- Wide Input Range: Robust performance across a broad range of starting material, from ultra-low 1 ng to standard 500 ng.
- Diverse Sample Types: Validated for high-performance results with genomic DNA (gDNA), Formalin-Fixed Paraffin-Embedded (FFPE) DNA, and cell-free DNA (cfDNA).
- Optimized for Target Enrichment: Fully compatible with liquid-phase hybridization capture workflows, providing the high library complexity needed for successful enrichment.
In the world of NGS, your data is only as good as your library. The LeXPrep DNA Library Preparation Module v2 removes the guesswork from sample prep, delivering the stability needed for routine sequencing and the sensitivity required for challenging samples like FFPE and cfDNA. Whether you are scaling up WGS or fine-tuning a targeted panel, LeXPrep v2 is the engine that powers your discovery.
| Package | Color Of Tube Cap | Component | Volume (24 rxn) | Volume (96 rxn) |
| Box 1 | ● | End Repair & A-Tailing Buffer Pro | 180 µL | 690 µL |
| ● | End Repair & A-Tailing Enzyme Pro | 120 µL | 460µL | |
| ● | Ligation Buffer | 750 µL | 2×1,500 µL | |
| ● | DNA Ligase | 65 µL | 250 µL | |
| ● | 2X HiFi PCR Master Pro Mix | 720 µL | 2×1,600 µL | |
| / | Nuclease Free Water | 2.5 mL | 8 mL | |
| ● | TE Solution | 1 mL | 4 mL | |
| Box 2 | / | LeXPrep SP Beads | 4 mL | 15 mL |
FFPE DNA is often fragmented and chemically modified. The LeXPrep v2 chemistry has been specifically tuned to be more “forgiving” of these imperfections, ensuring that even damaged fragments are efficiently ligated and amplified, leading to fewer gaps in your sequencing data.
Yes. The core chemistry remains consistent; you simply pair the module with the appropriate LeXPrep adapter kit designed for your specific sequencing platform.
Absolutely. Due to its high conversion efficiency and ability to maintain library complexity from low inputs, the LeXPrep v2 module is an ideal choice for the trace amounts of DNA typically recovered in liquid biopsy applications.
| Product | Catalog |
|---|---|
| LeXPrep DNA Library Preparation Module v2, 24 rxn | LX02421 |
| LeXPrep DNA Library Preparation Module v2, 96 rxn | LX02422 |
For inquiries or to request a formal quotation, please contact us at support@lexigenbio.com