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The Power of End-to-End LP-WGS for PGT-A

Precision in Every Embryo: Elevating PGT-A with LexigenBio’s End-to-End LP-WGS Solution

By LexigenBio Scientific Team

In the journey of In Vitro Fertilization (IVF), the transition from embryo biopsy to a successful pregnancy is a path paved with critical decisions. At the heart of these decisions is Preimplantation Genetic Testing for Aneuploidy (PGT-A). By identifying chromosomal abnormalities prior to uterine transfer, PGT-A has become a cornerstone of modern reproductive medicine, significantly improving implantation rates and reducing the risk of miscarriage.

As the industry moves away from older technologies like FISH and aCGH, Low-Pass Whole-Genome Sequencing (LP-WGS) has emerged as the gold standard for its superior resolution and accuracy. Today, LexigenBio is proud to present a fully integrated, end-to-end solution designed specifically for this high-stakes clinical application.

The Evolution of PGT-A: Why LP-WGS?

The primary goal of PGT-A is to identify whole-chromosome aneuploidies, segmental deletions/duplications, and mosaicism. While previous methods offered a glimpse into the embryonic genome, LP-WGS provides a comprehensive panoramic view.

By leveraging the power of Next-Generation Sequencing (NGS), LexigenBio’s solution delivers:

  • Higher Resolution: Detection of segmental abnormalities down to 5 Mb.
  • Mosaicism Sensitivity: Reliable calling of low-level mosaicism (approximately 30%).
  • Clinical Confidence: 100% concordance with known chromosomal standards (T13, T18, T21).

The LeXPrep Engine: From Single Cell to Library in 3 Hours

The biggest challenge in PGT-A is the starting material: typically just 1 to 10 trophectoderm cells. LexigenBio solves this through the LeXPrep 4S Strategy—Sensitivity, Speed, Stability, and Simplicity.

1. Single-Tube Simplicity

Traditional methods involve multiple tube transfers, which increase the risk of sample loss and exogenous DNA contamination. Our single-tube protocol preserves every picogram of embryonic DNA, ensuring a robust representation of the whole genome.

2. Industry-Leading Speed

Time is critical for fresh embryo transfers. The LeXPrep Single Cell WGA Kit completes amplification and library construction in under 3 hours, allowing clinical labs to move from “Sample to Insight” in a single shift.

3. Low Allele Dropout (ADO)

By utilizing optimized buffers and high-fidelity enzymes, we achieve ADO rates <10%, significantly outperforming traditional MDA and MALBAC methods. This precision is vital for the accurate calling of segmental aneuploidies.

Beyond the Reagents: The LeXee Bioinformatics Advantage

Raw data is only as good as the analysis behind it. LexigenBio’s end-to-end solution includes the LeXee Bioinformatics Visual System and the SenS PGT-A pipeline.

This automated system transforms complex sequencing reads into intuitive Copy Number Variation (CNV) plots, enabling clinicians to easily identify:

  • Whole-chromosome gains and losses.
  • Focal segmental deletions/duplications.
  • Mosaicism patterns across the 24-chromosome set.

Benchmarking Success: Outperforming the Competition

In rigorous benchmarking against industry leaders, the LexigenBio PGT-A solution demonstrated higher stability and superior resolution for segmental aneuploidies. Whether your lab is screening for common trisomies or investigating complex structural rearrangements, LexigenBio provides the technical edge required for clinical excellence.

Empowering Your Clinical Path

LexigenBio is dedicated to making high-fidelity PGT-A accessible and automated. By integrating advanced WGA chemistry with sophisticated bioinformatics, we offer a reliable, reproducible path to improving IVF outcomes.

Ready to elevate your PGT-A success? [Request a Consultation] | [End-to-End-Solution-for-PGT-A-by-LP-WGS] | [Contact support@lexigenbio.com]

Ordering Information:

  • LX30101: LeXPrep Single Cell WGA Kit (24 rxn)
  • LX02601: LeXPrep EZ DNA Library Preparation Module v2 (24 rxn)
  • LX09101: LeXee Bioinformatics Visual System
  • LX09102: Prebuilt Pipelines (SenS PGT-A)

Visit www.lexigenbio.com for more information.

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