Synchronous Detection of Methylation and Mutation with LeXDual
Beyond the Sequence: Synchronous Detection of Methylation and Mutation with LeXDual
By LexigenBio Scientific Team
In the rapidly evolving landscape of liquid biopsy and oncology research, DNA methylation has emerged as a powerhouse biomarker. Unlike traditional genetic mutations, methylation markers often appear earlier in cancer progression and provide greater precision for early diagnosis and minimal residual disease (MRD) monitoring.
However, a persistent challenge remains: How do we capture both the genetic (mutation) and epigenetic (methylation) landscape simultaneously from a single, ultra-low input sample?
Traditional bisulfite (BS) conversion is notoriously harsh, leading to significant DNA degradation. Enzymatic alternatives, while milder, can be cumbersome and unstable. To bridge this gap, LexigenBio is proud to introduce the LeXDual Comprehensive Solution.
The Innovation: MSRE Meets the LeXso Hybrid System
The LeXDual Comprehensive Solution integrates Methylation Sensitive Restriction Endonuclease (MSRE) technology with our exclusive LeXso Hybrid System. This synergy allows for the ultra-sensitive, synchronous detection of methylation and mutations without the need for destructive chemical conversion.
How it Works:
- Selective Cleavage: The LeXPrep Epicut Module utilizes MSRE to specifically cleave unmethylated sites. If a site is methylated, cleavage is blocked.
- Library Generation: This approach simultaneously generates enzyme-digested (unmethylated) and undigested (methylated) libraries from the same sample.
- Targeted Capture: Utilizing the LeXDual Panel, the system normalizes depth across MSRE sites and evaluates cleavage efficiency with high precision.
Why LeXDual is a Game-Changer for Multi-Omics
1. Preservation of Original Sequence Information
By avoiding bisulfite conversion, LeXDual preserves the full diversity and integrity of the original DNA sequence. This leads to Maximized Data Utilization and significantly higher effective coverage depth compared to traditional BS-based methods.
2. Ultra-High Sensitivity for Rare Signals
Detecting ultra-low-frequency mutations (down to 0.01% AF) and low-abundance methylation signals is critical for early detection. LeXDual enhances signal detection by maintaining high library complexity, making it ideal for trace cfDNA samples.
3. Streamlined Clinical Workflow
What used to take multiple reactions and sets of probes is now consolidated.
- One Sample
- One Hybridization Reaction
- One Set of Capture Probes
- Results in a Single Day
Performance in the Clinic: Differentiating Tumor from Healthy Populations
In clinical validations involving colorectal cancer patients, the LeXDual solution provided clear differentiation between tumor-derived cfDNA and healthy controls through Z-score analysis and precise methylation profiling.
Empowering the Next Generation of Oncology Research
The LeXDual Comprehensive Solution is more than just a kit; it is an end-to-end ecosystem. From the LeXPrep DNA Library Preparation Kit v2 to customized LeXDual Panels and specialized bioinformatics analysis, we provide the tools needed to turn limited samples into panoramic genomic insights.
Whether you are focusing on pan-cancer screening, MRD monitoring, or developmental biology, LeXDual offers the sensitivity and simplicity required to advance your research.
Ready to elevate your multi-omics research? [Request a Quote] | [LeXDual_Comprehensive Solution] | [Contact support@lexigenbio.com]
Ordering Information Highlights:
- LX02811: LeXPrep Epicut Module (24 rxn)
- LX15201: LeXso Hybrid Capture Reagents v2 (96 rxn)
- LX11340: LeXDual Beta Panel (6 rxn)
Visit www.lexigenbio.com for full product details.